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gfp signals  (Revvity)
96
Revvity gfp signals
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Gfp Signals, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaging based viability assay lentiviral particles encoding gfp  (Addgene inc)
93
Addgene inc imaging based viability assay lentiviral particles encoding gfp
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Imaging Based Viability Assay Lentiviral Particles Encoding Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gfp imaging  (Coherent Corp)
98
Coherent Corp gfp imaging
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Gfp Imaging, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell imaging  (Addgene inc)
93
Addgene inc cell imaging
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Cell Imaging, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse imaging include chicken anti gfp  (AvesLabs)
98
AvesLabs mouse imaging include chicken anti gfp
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Mouse Imaging Include Chicken Anti Gfp, supplied by AvesLabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine imaging include chicken anti gfp  (AvesLabs)
98
AvesLabs murine imaging include chicken anti gfp
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Murine Imaging Include Chicken Anti Gfp, supplied by AvesLabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine imaging include chicken anti gfp - by Bioz Stars, 2026-04
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gfp channel of an evostm fl auto imaging system  (Thermo Fisher)
90
Thermo Fisher gfp channel of an evostm fl auto imaging system
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Gfp Channel Of An Evostm Fl Auto Imaging System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phase contrast and gfp/fluorescence microscopy images  (KEYENCE)
90
KEYENCE phase contrast and gfp/fluorescence microscopy images
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Phase Contrast And Gfp/Fluorescence Microscopy Images, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imag-min/gfp measuring head  (Heinz Walz)
90
Heinz Walz imag-min/gfp measuring head
a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following <t>subcutaneous</t> <t>inoculation</t> of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl <t>(Ctrl)-GFP,</t> sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.
Imag Min/Gfp Measuring Head, supplied by Heinz Walz, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following subcutaneous inoculation of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl (Ctrl)-GFP, sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.

Journal: Nature Communications

Article Title: IFN-γ-driven UBE2D3 upregulation impairs antigen presentation pathways and anti-tumor immunity in pancreatic cancer

doi: 10.1038/s41467-025-65762-4

Figure Lengend Snippet: a Hypothesis diagram depicting the crosstalk between immune inflammatory cells and PDAC cells. b Heatmap of transcriptome DEGs in the cellular response to IFN-γ process between KPC-shScramble (Scr) cells and KPC-shUbe2d3-1 (KD1) cells ( n = 3 samples per group). c Tumor growth curves following subcutaneous inoculation of KPC/Panc02 Scr/KD1 cells in syngeneic immunodeficient Rag1 −/− mice and subsequent implantation into immunocompetent C57BL/6 mice. Once tumor reached 1000 mm 3 in Rag1 −/− mice ( n = 4 mice per group), they were excised, and 1–2 mm 3 fragments were further inoculated subcutaneously into immunocompetent C57BL/6 mice ( n = 7 mice per group). d Experimental setup for in vitro CTL-mediated killing of KPC-WT/KO cells. KPC-WT cells were subjected to lentiviral transduction with the indicated sgCtrl (Ctrl)-GFP, sgCtrl (Ctrl)-mCherry, sgUbe2d3-1 (KO1)-mCherry, or sgUbe2d3-2 (KO2)-mCherry Cas9 vectors. These cells were further transiently transfected with the OVA OE pcDNA3 plasmid to construct target cells. OT-I CD8 + T cells were cocultured with either a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -Ctrl-mCherry cells or a mixture of KPC OVA -Ctrl-GFP cells and KPC OVA -KO-mCherry cells for 24 h ( n = 5 independent experiments per group). e Quantification of tumor-infiltrating CD4 + T cells and CD8 + T cells in subcutaneous KPC Scr/KD1 model ( n = 5 mice per group) and Panc02 Scr/KD1 model ( n = 9 mice per group). f Tumor growth curves from CD4 + or CD8 + T-cell deletion assay ( n = 5 mice per group). g Tumor growth evaluation in humanized NCG mice. Seven days post-PBMC injection, these immunoreconstituted mice were subcutaneously engrafted with Capan-1-Scr or Capan-1-KD1 cells. The level of immune humanization was confirmed by flow cytometric analysis of hCD45 + cells in peripheral blood on days 7 and 21. All mice were sacrificed 24 days after tumor inoculation for tumor weight measurement ( n = 7 mice per group). Statistical significance was determined via the unpaired t test (two-tailed) in ( c – g ). The data are presented as the means ± SDs. Source data are provided in the Source Data file.

Article Snippet: The tumor burdens in the pancreas and liver were examined 21 d after tumor inoculation via IVIS detection of GFP signals (PerkinElmer, Lumina Series III).

Techniques: In Vitro, Transduction, Transfection, Plasmid Preparation, Construct, DNA Deletion Assay, Injection, Two Tailed Test